FREEZE QUENCH
Accessory to enable the conversion of stopped-flow / quench-flow to a freeze-quench.
When chemical quenching is not suitable for a rapid kinetics study, fast freezing the reaction to quench it may be the best option. Freeze quench experiments are divided into three phases: mixing, ageing and freezing. The frozen reaction products are collected and analyzed by EPR/NMR, Mossbauer or XAFS in their solid state. Freeze quench can be used to study full and intermediate kinetics by trapping reaction intermediates, whose presence has been determined by stopped-flow experiments. Freeze quench techniques are also extensively used to study metallic reaction centers in metalloproteins and metalloenzymes.
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SFM accessories(1.3 MB)
Accessories necessary for different measurement techniques
